These experiment were conducted to investigate the cultural condition of the lipase production by Rhizopus japonicus. The results obtained were as follows:
1. Saybean meal and ammonium sulfate were the most effective in the lipase production as organic and inorganic nitrogen sources, respectively.
2. The lipase production was strongly inhibited, when added as carbon sources aylose, glucose, fructose, galactose, maltose, soluble starch, and dextrin causing the lowering of pH of the medium during culture. Sucrose did not inhibit the lipase production, but not caused any effect when added.
3. K₂HPO₄ as phosphate salt and MgSO₄·H₂O as magnesium salt were the most effective is the lipase production.
4. The addition of olive oil, soybean oil, and coconut oil respectively increased the enzyme production and especially 1% olive oil increased it by 50%.
5. The enzyme production increased slightly on the addition of yeast extract to 0.05∼0.07%.
6. The optimum composition of the medium for the lipase production by Rhizopus japonicas was in the compositioa of soybean meal 2%; K₂HPO₄ 0.5%; (NH₄)₂SO ₄
0.1%; MgSO₄·7H₂O 0.05%; yeast extract 0.05%; olive oil 1%. The maximum production of the lipase was attained by the incubation far 48hrs under the optimum incubation condition.
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